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目的:探讨大黄素抗人乳腺癌MCF-7细胞增殖及其相关机制。方法:采用MTT法检测大黄素对MCF-7细胞的增殖抑制作用;流式细胞术分析周期分布和凋亡情况;原子力显微镜(AFM)观察细胞膜表面超微结构的变化。结果:大黄素呈剂量依赖性地抑制MCF-7细胞的增殖;大黄素能使MCF-7细胞阻滞在G0/G1期;Annexin V/PI双染法结果表明,大黄素对MCF-7细胞没有明显的促凋亡作用。AFM观察细胞膜超微结构,结果显示对照组细胞核区饱满,膜表面平坦光滑;大黄素作用48 h可致细胞核区坍塌萎缩,细胞表面颗粒密集,膜表面的平均粗糙度(Ra)和均方粗糙度(Rq)与对照组相比均有显著增加(P<0.05)。结论:大黄素通过阻断MCF-7细胞的细胞周期进程及影响细胞膜超微结构而发挥抗肿瘤作用。
Objective: To investigate the proliferation of emodin anti-human breast cancer cell line MCF-7 and its related mechanism. Methods: The inhibitory effect of emodin on the proliferation of MCF-7 cells was detected by MTT assay. The cell cycle distribution and apoptosis were analyzed by flow cytometry. The ultrastructure of cell membrane was observed by atomic force microscopy (AFM). Results: Emodin inhibited the proliferation of MCF-7 cells in a dose-dependent manner. Emodin inhibited the proliferation of MCF-7 cells in G0 / G1 phase. The results of Annexin V / PI double staining showed that emodin inhibited the proliferation of MCF- No obvious pro-apoptotic effect. AFM observed the ultrastructure of the cell membrane, the results showed that the control group of nuclear area full, smooth and smooth membrane surface; emodin 48h can cause collapse of the nucleus collapsed, the cell surface dense particles, the membrane surface roughness (Ra) and mean square roughness Degree (Rq) was significantly increased compared with the control group (P <0.05). Conclusion: Emodin can exert antitumor effect by blocking the cell cycle progression and influencing the ultrastructure of MCF-7 cells.