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应用自行设计的装置，使圆指额蚤上位亚种、无棘额蚤、秃病蚤田鼠亚种、斧形盖蚤和谢氏山蚤感染鼠疫菌（EV76、A1122和91001株）。五种蚤感染程序相同。在温度为16±2℃、20±2C、24±2℃和相对湿度为65±5％的条件下，蚤吸入每毫升含有103个菌的兔血、羊血、猪血后1．5小时，将蚤制成悬液检菌。以兔血为供血源，应用91001株，在24±2℃环境组中的蚤感染率（45．52％）比其他环境组高。蚤吸血后的第2～35天，在蚤前胃中未发现有菌栓形成。
The Yersinia pestis (EV76, A1122 and 91001) was infected by the self-designed device, which was used to induce the ephemeral fleas, the unrivaled flea, the vole subsp. The five flea infection procedures are the same. At a temperature of 16 ± 2 ° C, 20 ± 2 ° C, 24 ± 2 ° C and a relative humidity of 65 ± 5%, fleas inhaled 1.03 ml of rabbit blood, sheep blood, and porcine blood per 103 ml , The flea made suspension of bacteria. Using rabbit blood as the blood supply source, 91001 strains were used, and the flea infection rate (45.52%) in the environment group of 24 ± 2 ℃ was higher than that in other environment groups. 2 ~ 35 days after fleas vampire, there was no bacterial plug formation in the anterior stomach of fleas.