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为了探讨雌激素受体(estrogen receptor,ESR)、骨桥蛋白(osteopontin,OPN)和视黄醇结合蛋白4(retinol binding proteins 4,RBP4)的多态性对大白猪繁殖性状的影响,本研究采用PCR-RFLP技术检测试验猪群ESR、OPN和RBP4基因的多态性,并分析其对大白猪总产仔数、产活仔数、健仔数、窝重、初生重、死胎、木乃伊、弱仔数和畸形数等性状的影响。结果显示,在大白经产母猪中,ESR和OPN基因的优势基因为A等位基因,RBP4基因的优势基因为B等位基因;ESR基因AB与BB基因型在总产仔数、产活仔数性状上均高于AA基因型;OPN基因在总产仔数性状上AA基因型最高,在产活仔数、健仔数、窝重性状上均为BB>AA>AB;RBP4基因在总产仔数、产活仔数、健仔数、窝重性状上均为AA>BB>AB;合并基因型分析发现ESR-RBP4最优的分子标记组合为ABAA;OPN-RBP4最优的分子标记组合为BBAA。因此,ESR、OPN和RBP4基因多态性与大白猪繁殖性状相关。
In order to investigate the effects of estrogen receptor (ESR), osteopontin (OPN) and retinol binding proteins 4 (RBP4) polymorphisms on reproductive traits in Landrace, The polymorphisms of ESR, OPN and RBP4 gene in pigs were detected by PCR-RFLP. The total number of piglets born alive, the number of live born, the number of adolescent, litter weight, birth weight, stillbirth, mummies, Weak number and number of malformations and other traits. The results showed that the dominant allele of ESR and OPN genes was A allele and the dominant gene of RBP4 gene was B allele in sows of Dabai sow; The number of AA genotypes was higher than that of AA genotypes. The genotypes of OPN gene were the highest in the total number of litter size, BB> AA> AB in the number of alive pups, litter size and litter weight; Total litter size, live litter size, litter size and litter weight were all AA> BB> AB. The pooled genotypes of ESR-RBP4 were ABAA and OPN-RBP4 was the best Tag combination for BBAA. Therefore, the polymorphisms of ESR, OPN and RBP4 are related to reproductive traits in Landrace.