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目的:研究幽门螺杆菌(Helicobacter pylori,Hp)代谢产物对炎性细胞因子白细胞介素8(interleukin-8,IL-8)表达的影响以及在胃粘膜上皮细胞增殖中的作用。方法:用CagA阳性Hp培养滤液给大鼠灌胃,2周后,采用ELISA法检测鼠血清中的IL-8水平,免疫组织化学SP(streptavidin/perosidase)法检测胃粘膜增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)的表达。结果:灌服Hp滤液组与对照组比较,血清IL-8升高,胃粘膜PCNA标记指数阳性率升高,均有显著性意义。结论:CagA阳性Hp培养滤液可促进炎性因子IL-8的表达,促进胃粘膜上皮细胞的增殖,可能促进胃癌的发生。
Objective: To investigate the effects of Helicobacter pylori (Hp) metabolites on the expression of inflammatory cytokines interleukin-8 (IL-8) and their roles in the proliferation of gastric mucosal epithelial cells. Methods: The rats were inoculated intragastrically with CagA positive Hp culture filtrate. After 2 weeks, the level of IL-8 in serum was detected by ELISA, and the proliferation cell proliferation was detected by streptavidin / perosidase (SP) nuclear antigen, PCNA) expression. Results: Compared with the control group, the serum IL-8 level was increased and the positive rate of PCNA marker index in gastric mucosa was significantly increased. Conclusion: CagA-positive Hp culture filtrate can promote the expression of IL-8, promote the proliferation of gastric mucosal epithelial cells, and may promote the occurrence of gastric cancer.