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目的 研究绞股蓝总皂苷（ Ｇｙｐ） 对血管平滑肌细胞增殖的调节与内皮细胞ｃｓｉｓ 基因表达和一氧化氮（ Ｎ Ｏ） 合成／ 释放的关系。方法 采用原位杂交法检测内皮细胞ｃｓｉｓｍ Ｒ Ｎ Ａ 表达，用 Ｇｒｉｅｓｓ 法测定内皮细胞 Ｎ Ｏ 释放量，用 Ｍ Ｔ Ｔ快速比色法检测平滑肌细胞（ Ｓ Ｍ Ｃ） 增殖。结果 内毒素（ Ｌ Ｐ Ｓ）１６ ｍｇ· Ｌ－ １ 促进牛主动脉内皮细胞ｃｓｉｓ 基因表达，明显降低内皮细胞培养液中一氧化氮含量，刺激 Ｓ Ｍ Ｃ 增殖；黄嘌呤－ 黄嘌呤氧化酶（ Ｘ Ｘ Ｏ） 系统产生的氧自由基（ Ｏ Ｆ Ｒ） 具有与 Ｌ Ｐ Ｓ 相似的作用。绞股蓝总皂苷对 Ｌ Ｐ Ｓ诱导的内皮细胞ｃｓｉｓ 基因表达具有明显抑制作用，并能对抗 Ｏ Ｆ Ｒ 诱导的 Ｅ Ｃ 损伤，保护内皮细胞释放 Ｎ Ｏ 的能力，抑制 Ｌ Ｐ Ｓ 和 Ｏ Ｆ Ｒ通过 Ｅ Ｃ 介导的 Ｓ Ｍ Ｃ 增殖。 Ｇｙｐ 的效应可被 Ｎ Ｏ 合成酶抑制剂硝基左旋精氨酸部分取消。结论 绞股蓝总皂苷通过抑制内皮细胞ｃｓｉｓ 基因表达，促进内皮细胞合成（ 释放） Ｎ Ｏ抑制平滑肌细胞增殖
Objective To study the relationship between the regulation of vascular smooth muscle cell proliferation and the gene expression of csis endothelial cells and the synthesis / release of nitric oxide (NO) by Gyp. Methods The in situ hybridization was used to detect the expression of csism R N A in endothelial cells. The amount of N O released by endothelial cells was measured by Griess method. The proliferation of smooth muscle cells (SMCs) was detected by MTT colorimetry. Results Endotoxin (L P S) 16 mg · L -1 promoted the expression of csis gene in bovine aortic endothelial cells, significantly reduced the content of nitric oxide in endothelial cell culture medium and stimulated the proliferation of S M C. The xanthine-xanthine oxidation Oxygen free radicals (O F R) produced by the enzyme (X X O) system have a similar effect as L P S. Gypenosides on L P S induced endothelial cell c sis gene expression was significantly inhibited, and O F R-induced E C injury, the protection of endothelial cells release N O ability, inhibition of L P S and O F R ECM-mediated S M C proliferation. The effect of Gyp can be partially abolished by the NOase inhibitor nitro-L-arginine. Conclusion Gypenosides can inhibit endothelial cell csis gene expression and promote the synthesis of endothelial cells (release) N O inhibition of smooth muscle cell proliferation