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以１６种重要的籼稻和粳稻栽培品种为材料，研究了影响农杆菌转化水稻频率的有关因素。结果表明：ＣＣ培养基是绝大多数水稻愈伤组织的最适诱导与继代培养基；添加２．５～５ｍｇ ／ Ｌ ＡＢＡ可以有效地改善水稻愈伤组织的质量；籼稻愈伤组织所需的筛选剂浓度低于粳稻愈伤组织所需的浓度；根癌农杆菌ＥＨＡ１０５菌株对水稻的转化效果优于ＬＢＡ４４０４和ＡＧＬ１菌株的效果；头孢霉素对农杆菌的抑制效果优于羧苄青霉素的效果；共培养后进行适当的干燥处理既可增强脱菌效果，又可提高转化频率。应用我们所优化的农杆菌转化技术体系，获得了１０个品种的水稻转基因植株。
Sixteen important indica and japonica cultivars were used as materials to study the factors influencing the Agrobacterium - mediated transformation of rice. The results showed that: CC medium was the most suitable induction and subculture medium for most callus; addition of 2.5 ~ 5 mg / L ABA could effectively improve the quality of rice callus; the callus of indica rice Was lower than that of japonica callus. The transformation efficiency of Agrobacterium tumefaciens strain EHA105 was better than that of LBA4404 and AGL1 strains. The inhibitory effect of cefotaxime on Agrobacterium was better than that of carbenicillin Effect; co-culture after proper drying can enhance the effect of sterilization, but also improve the conversion frequency. Using our optimized Agrobacterium transformation technology system, 10 varieties of transgenic rice plants were obtained.