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从５１种豆科树种（含羞草亚科５属，蝶形花亚科１８属）根瘤中分离获得的６６个菌株与４种豆科植物（刺槐、合欢、大豆、豌豆）进行接种试验，幼苗经５０ｄ培养，刺槐、合欢、大豆、豌豆的结瘤率分别为１００％、８７．９％，６９．７％、２５．８％。其中有１２个菌株可同时在这４种豆科植物上结瘤，２９个菌株可同时在刺槐、合欢和大豆上结瘤，４个可同时在刺槐、合欢和豌豆上结瘤，说明豆科树种根瘤菌具有较广泛的共生寄主范围。各菌株与不同豆科植物的亲合力、结瘤特性和固氮能力有较明显差异。观察还进一步证实根瘤的形状和颜色是由寄主植物决定的。
Sixty-six strains isolated from the root nodules of 51 leguminous species (5 genera of Mimosaceae and 18 genera of Papilio) were inoculated with 4 legumes (acacia, acacia, soybean and pea) After 50 days, the nodulation rates of R. pseudoacacia, Acacia, soybean and pea were 100%, 87.9%, 69.7% and 25.8% respectively. Twelve of these strains were able to nodulate on these four legumes at the same time. Nineteen strains could simultaneously nodulate locust, acacia and soybean, and four nodules could simultaneously nodulate on locust, acacia and pea, indicating that legume Rhizobium species have a wider range of commensal host. The avidity, nodulation characteristics and nitrogen fixation ability of each strain were significantly different from those of other leguminous plants. Observation also further confirmed that the nodule shape and color is determined by the host plant.