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目的 了解Ca2 + ATP酶 (即钙泵 )在耳蜗活动位点及内淋巴积水后的变化 ,测复合动作电位 (CAP)与Ca2 + ATP酶的活性关系。方法 健康豚鼠 14只 ,左耳圆窗装电极后测CAP阈值正常者阻塞内淋巴囊 ,使诱发内淋巴积水。对照耳和积水耳进入细胞组化试验。对Ca2 + ATP酶活性用枸橼酸铅反应进行细胞化学研究 ,在电镜下以磷酸颗粒表示Ca2 + ATP酶活性。结果 Ca2 + ATP酶活跃在蜗管前庭膜内淋巴侧、内外毛细胞之皮板及静纤毛、血管纹边缘细胞内浆膜等处 ;内淋巴积水后 ,滤波短声复合动作电位阈值增高 ,前庭膜、内外毛细胞等处之Ca2 + ATP颗粒明显减少。结论 内淋巴积水后CAP阈值提高 ,前庭膜、内外毛细胞等处Ca2 + ATP酶活性明显下降 ,两者呈负相关。
Objective To investigate the changes of Ca2 + ATPase (calcium pump) after cochlear activity and endolymphatic hydrops, and to determine the relationship between the compound action potential (CAP) and Ca2 + ATPase activity. Methods Fourteen healthy guinea pigs were enrolled, and the threshold of CAP was measured after left ear round window was fitted with electrodes to block the endolymphatic sac, which induced endolymphatic hydrops. Control ear and water ear into the cell group test. Cytochemical study of Ca2 + ATPase activity with lead citrate showed that Ca2 + ATPase activity was represented by phosphoric acid particles under electron microscope. Results Ca2 + ATPase was active in the lymphatic wall of the vestibular membrane of the cochlea, the dermis and the cilia of the inner and outer hair cells, the serous membrane of marginal cells in the stria vascularis. After the endolymphatic hydrops, the threshold value of the compound action potential of the filtering short tone increased, Vestibular membrane, inside and outside the hair cells at the Ca2 + ATP particles significantly reduced. Conclusions The CAP threshold increases after endolymphatic hydrops and the activity of Ca2 + ATPase in the vestibular membrane, inner and outer hair cells and so on obviously decreases, and the two are negatively correlated.