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目的探讨高危型人乳头瘤病毒(HPV)E6/E7 mRNA在宫颈癌筛查中的应用性能及意义。方法选择2014年1月至2016年1月濮阳市妇幼保健院行液基薄层细胞学检测(TCT)结果为阳性且均进行了HPV DNA分型、HPVE6/7mRNA及组织学检测的206例患者为研究对象,以组织学检测为基准,对HPV DNA分型、HPVE6/7mRNA检测两种方法进行统计学分析。结果 206例患者HPV DNA分型、HPVE6/7mRNA检测的阳性率分别为73.8%、67.5%,两者无统计学意义(P>0.05);敏感度分别为83.2%、78.3%,两者无统计学意义(P>0.05);特异度分别为60.7%、87.65%,具统计学差异(P<0.05);且HPVE6/7mRNA检测的约登指数(65.95%)高于HPV DNA分型检测(43.9%)。结论 HPV E6/E7 mRNA检测在宫颈癌筛查中特异度及约登指数较高,对于进行宫颈癌筛查的患者来说价值更高。
Objective To investigate the application and significance of high-risk human papillomavirus (HPV) E6 / E7 mRNA in cervical cancer screening. Methods From January 2014 to January 2016, 206 patients with positive HPV genotyping, HPVE6 / 7 mRNA and histological examination in Puyang City Maternal and Child Health Hospital were detected by TCT. As the research object, based on the histological examination, the HPV DNA typing and HPVE6 / 7 mRNA testing were statistically analyzed. Results The positive rates of HPV DNA typing and HPVE6 / 7 mRNA in 206 patients were 73.8% and 67.5%, respectively, with no significant difference (P> 0.05). The sensitivity was 83.2% and 78.3% respectively (P> 0.05). The specificities were 60.7% and 87.65%, respectively, with statistical significance (P <0.05). The Youden Index (65.95%) of HPVE6 / 7 mRNA was higher than that of HPV DNA typing %). Conclusion The specificity of HPV E6 / E7 mRNA detection in cervical cancer screening and higher Youden index are more valuable for cervical cancer screening.