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目的:研究脑干交感运动前区的前阿黑皮素能神经纤维的分布及其传入投射。方法:以免疫组化法检测前阿黑皮素能神经纤维在脑干交感运动前区的分布;将霍乱毒素B微量注入小鼠脑干交感运动前区进行逆行追踪。结果:α-MSH(α-melanocyte-stimulating hormone)能神经终末密集分布于交感运动前区内,同时则未见AgRP(agouti-related protein)能神经终末,显示交感运动前区仅接受前阿黑皮素(POMC)能神经的单向调节。逆行追踪实验显示脑干交感运动前区的POMC能纤维投射来自于下丘脑弓状核/视交叉后区,而非孤束核尾侧部;统计结果表明下丘脑弓状核/视交叉后区向脑干交感运动前区的神经投射中有近一半为POMC能投射。结论:小鼠下丘脑的POMC能神经元可直接投射到脑干交感运动前区,该神经通路在能量平衡的调节中可能发挥重要的作用。
OBJECTIVE: To study the distribution and projectile projection of pro-melanocortin nerve fibers in the pre-sympathetic area of brain stem. Methods: Immunohistochemistry was used to detect the distribution of pro-melanocortin nerve fibers in the presympathetic area of brainstem. The cholera toxin B was injected into the anterior area of mouse brain stem in the sympathetic movement for retrograde tracing. Results: α-MSH (α-melanocyte-stimulating hormone) can be localized in the pre-sympathetic area without any AgRP (agouti-related protein) One-way regulation of melanocortin (POMC) can nerves. Retrograde tracing experiments showed that the POMC fibers in the anterior brainstem sympathetic area were projected from the hypothalamic arcuate nucleus / optic chiasm but not the caudal part of the nucleus tractus solitarius. The statistical results showed that the hypothalamic arcuate / optic chiasm posterior Nearly half of the neuronal projections to the brain stem sympathetic anterior area are POMC projections. CONCLUSION: POMC neurons in the hypothalamus of mice can be directly projected into the presympositic region of brain stem, which may play an important role in the regulation of energy balance.