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目的:探讨明胶酶的表达与鼻咽癌细胞转移潜能的关系。方法:Real-time PCR和明胶酶谱法检测CNE1、CNE1-LMP1、CNE2、SUNE1-5-8F和SUNE1-6-10B 5株鼻咽癌细胞株中明胶酶mRNA的表达及分泌,并比较其差异。结果:明胶酶mRNA在5株鼻咽癌细胞中都有表达,其相对表达量为SUNE1-5-8F>CNE1-LMP1>SUNE1-6-10B>CNE2>CNE1,其中,明胶酶mRNA在SUNE1-5-8F细胞和SUNE1-6-10B之间以及CNE1-LMP1细胞和CNE1细胞之间的表达差异有统计学意义,P<0.001;在CNE2细胞和CNE1细胞之间,MMP-9 mRNA的表达差异有统计学意义(P<0.001),而MMP-2 mR-NA的表达差异无统计学意义,P=0.078;明胶酶谱结果显示,明胶酶的活性在CNE1-LMP1细胞上清液中明显高于CNE1细胞(P≤0.001),在SUNE1-5-8F细胞上清液中明显高于SUNE1-6-10B细胞(P<0.001),而CEN2细胞分泌明胶酶的量为5株细胞中最低1株。结论:明胶酶的表达和分泌与鼻咽癌细胞的转移潜能密切相关。
Objective: To investigate the relationship between the expression of gelatinase and the metastatic potential of nasopharyngeal carcinoma cells. Methods: The expression and secretion of gelatinase mRNA in CNE1, CNE1-LMP1, CNE2, SUNE1-5-8F and SUNE1-6-10B 5 NPC cell lines were detected by Real-time PCR and gelatin zymography difference. Results: Gelatinase mRNA was expressed in 5 NPC cells, and the relative expression levels of gelatinase mRNA were SUNE1-5-8F> CNE1-LMP1> SUNE1-6-10B> CNE2> CNE1, There was significant difference between the expression of 5-8F and SUNE1-6-10B and the expression of CNE1-LMP1 and CNE1 cells, P <0.001; between CNE2 cells and CNE1 cells, the difference of MMP-9 mRNA expression (P <0.001), while the expression of MMP-2 mR-NA had no statistical difference (P = 0.078). The results of gelatin zymography showed that the activity of gelatinase in CNE1-LMP1 cell supernatant was significantly higher In CNE1 cells (P≤0.001), SUNE1-5-8F supernatant was significantly higher than that of SUNE1-6-10B cells (P <0.001), whereas the amount of CEN2 cells secreting gelatinase was the lowest among 5 cells Strain. Conclusion: The expression and secretion of gelatinase are closely related to the metastatic potential of nasopharyngeal carcinoma cells.