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我们的工作是应用YF2PSK31R型超滤器超滤粗提基因工程干扰素,并与盐析法对比。超滤法简便快速条件温和,超滤后干扰素平均收率为93.95%±2.48%,盐析法则为75.93%±3.9%,前者比后者提高18.02%,差异显著。比活前为:4.455E6±7.58E5IU/mg蛋白,后者为:1.6117E6±2.079E5IU/mg蛋白,差异也是显著的
Our work is the application of YF2PSK31R ultrafiltration ultrafiltration crude genetic engineering interferon, and compared with the salting-out method. The ultrafiltration method was simple and rapid with mild conditions. The average yield of interferon was 93.95% ± 2.48% after ultrafiltration and 75.93% ± 3.9% by salting out method. The former was 18.02% ,Significant difference. The specific activity was: 4.455E6 ± 7.58E5IU / mg protein, the latter was: 1.6117E6 ± 2.079E5IU / mg protein, the difference was also significant