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目的：对抗原基因cC1进行结构分析和初步功能研究。方法：利用网上生物信息学软件进行cC1的一级结构、二级结构分析，同源建模预测三级结构。白陶土部分凝血活酶时间（KPTT）检测重组蛋白GST－anx32的抗凝血活性。结果：cC1在核酸水平和氨基酸水平均与膜联蛋白基因同源，具有4个同源结构区域，且每一个同源区域均具有膜联蛋白的典型motif“G－X－G－T（38 residues）－D／E”。抗凝血实验表明大肠杆菌表达的谷胱甘肽转移酶－cC1融合蛋白具有很强的抗凝血活性。结论；抗原基因cC1具有膜联蛋白家族的典型特征，但与已知的31个亚家族氨基酸序列的同源性均低于48％，因此是一个新的膜联蛋白亚家族成员，命名为膜联蛋白32（anx32）。为进一步研究其生理功能及核酸疫苗pcDNA3－γcC1诱导囊尾蚴细胞凋亡的分子机制打下了基础。
OBJECTIVE: To study the structure and primary function of antigenic gene cC1. Methods: The primary structure and secondary structure of cC1 were analyzed by online bioinformatics software, and the homology modeling was used to predict the tertiary structure. The anticoagulant activity of the recombinant protein GST-anx32 was assayed by kaolin partial thromboplastin time (KPTT). Results: cC1 was homologous with annexin gene at the level of nucleic acid and amino acid, and had 4 homologous structural regions, and each homologous region had a typical motif of annexin “G-X-G-T residues) -D / E ”. Anticoagulation experiments showed that E. coli expression of glutathione transferase-cC1 fusion protein has strong anticoagulant activity. CONCLUSION: The antigen gene cC1 has typical features of annexin family, but its homology with the known 31 subfamily amino acid sequences is less than 48%. Therefore, cC1 is a new member of the annexin subfamily named as membrane Cofactor 32 (anx32). Which lays the foundation for further study on the molecular mechanism of its physiological function and apoptosis of cysticercus cellulolytica induced by pcDNA3-γcC1.