论文部分内容阅读
目的观察麻杏石甘汤两种煎煮方法对哮喘模型小鼠气道平滑肌细胞转化生长因子(TGF-β1)、缺氧诱导因子1α(HIF-1α)的影响。方法将40只合格小鼠随机分为4组:正常对照组,哮喘模型组,麻杏石甘汤先煎组,麻杏石甘汤合煎组,每组10只。除正常对照组外其他各组均用鸡卵白蛋白(OVA)腹腔注射致敏小鼠,并以低浓度OVA雾化吸入激发制作哮喘模型。麻杏石甘汤先煎组、麻杏石甘汤合煎组从激发开始连续7天灌胃给药,分别测定各组小鼠支气管肺泡灌洗液TGF-β1及肺组织匀浆HIF-1α浓度。结果麻杏石甘汤先煎组在降低小鼠支气管肺泡灌洗液TGF-β1、肺组织匀浆HIF-1α浓度方面优于麻杏石甘汤合煎组(P<0.05)。结论麻杏石甘汤除去麻黄上沫的煎煮方法更安全,更有利于抑制哮喘气道平滑肌细胞增殖和改善缺氧状态。
Objective To observe the effects of Maxing Shigan Decoction on the expression of transforming growth factor (TGF-β1) and hypoxia-inducible factor-1α (HIF-1α) in airway smooth muscle cells of asthmatic mice. Methods Forty eligible mice were randomly divided into 4 groups: normal control group, asthma model group, Maxing Shigan Decoction group and Maxing Shigan Decoction group with 10 rats in each group. In addition to the normal control group other groups were sensitized with intraperitoneal injection of chicken ovalbumin (OVA) mice and aerosol inhalation challenge with low concentrations of OVA to produce asthma model. Maxing Shigan Decoction group and Maxing Shigan Decoction group were administrated intragastrically for 7 consecutive days. The levels of TGF-β1 in bronchoalveolar lavage fluid and HIF-1α concentration. Results Maxing Shigan Decoction group was better than Maxingshigan Decoction (P <0.05) in reducing the concentration of TGF-β1 in bronchoalveolar lavage fluid and the concentration of HIF-1α in lung homogenate. Conclusion Maxingshigan Decoction to remove ephedra on the foam decoction safer and more conducive to the inhibition of asthma airway smooth muscle cell proliferation and improve hypoxia.