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目的建立一种快速、敏感、特异的荧光定量PCR方法检测食品中的几种主要食源性致病菌。方法以沙门氏菌、单增李斯特氏菌、大肠杆菌O157：H7的特异性DNA核酸片段作为靶序列，采用聚合酶链反应(PCR)结合Taqman技术，以哈尔滨市2013~2014年度食源性致病菌主动监测分离鉴定的菌株作为阳性菌，对其进行荧光检测；并对其特异性和与传统检测方法的符合性进行评价。结果本研究建立的反应体系对8株沙门氏菌、19株单增李斯特氏菌、4株大肠杆菌O157：H7均呈现出良好的扩增曲线，表明该方法具有很好的特异性与符合性，与传统方法的复合率为100%。结论该方法特异性强、灵敏度高、适应于由食源性致病菌引起的食物中毒及食源性疾病的快速检测，具有广阔的应用前景及推广价值。“,”Objective To establish a rapid, sensitive, specific quantitative PCR method for detection several major food borne pathogen. Methods As Salmonel a, Listeria monocytogenes, Escherichia coliO157:H7specific DNA fragment as a target nucleic acid sequence, using the polymerase chain reaction (PCR) with Taqman technology, detected positive strains isolated from the 2013-2014 foodborne pathogens monitoring of Harbin City and assessed the specificity and the compliance with the traditional detection method. Results 8 strains of Salmonel a and 19 strains of Listeria bacteria List S, 4 strains of Escherichia coli O157:H7 showed good amplification curves of the reaction system ,it showed that the method had good specificity and coincidence rate with the traditional methods, the composite was 100%. Conclusion The method was strong specificity ,high sensitivity, which suited for rapid detection of food poisoning and food borne disease caused by pathogenic bacteria, and had wide application prospect and popularization value.