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在温室土壤灭菌盆栽条件下对黄瓜(Cucumis sativus Linn.,“中农26号”)接种丛枝菌根(arbuscular mycorrhizal,AM)真菌Funneliformis mosseae,测定了黄瓜根围土壤微生物功能、微生物数量以及土壤理化性质、土壤酶活性的变化。结果表明AM真菌可以提高土壤的速效养分含量,改善土壤物理性质,提高土壤中磷酸酶、氨肽基酶、多酚氧化酶及过氧化物酶活性;接菌处理后土壤中细菌、真菌数量显著高于不接种处理,而放线菌数量低于不接菌处理;对根围微生物功能多样性指标分析表明接菌处理的平均颜色变化率(AWCD)、Simpson优势度指数以及Mclntosh多样性指数,显著低于不接种对照。对菌根侵染率与土壤微生物等性状间的RDA分析表明AM真菌侵染率与速效P含量(r=0.65,P<0.05)、磷酸酶(r=0.74,P<0.05)、氨肽基酶(r=0.86,P<0.05)、真菌数量(r=0.57,P<0.05)呈正相关,与酚酸类碳源(r=-0.55,P<0.05)、胺类碳源(r=-0.58,P<0.05)、速效N(r=-0.77,P<0.05)、β-葡糖苷酶(r=-0.90,P<0.05)、β-纤维二糖苷酶(r=-0.63,P<0.05)呈负相关。因此接种AM真菌的黄瓜菌根苗种植提高了土壤酶活性,优化了温室黄瓜根围微生物群落结构,显著改善了土壤理化性质。
Cucumber (Cucumis sativus Linn., “Zhongnong 26”) was inoculated with Funneliformis mosseae, an arbuscular mycorrhizal (AM) fungus, and the effect of soil microbial function, And soil physical and chemical properties, changes in soil enzyme activity. The results showed that AM fungi could increase the available nutrient content of soil, improve the physical properties of soil, and increase the activities of phosphatase, aminopeptidase, polyphenol oxidase and peroxidase in soil. After inoculation, the number of bacteria and fungi in soil was significant The results showed that the average color change rate (AWCD), Simpson dominance index and Mclntosh diversity index of inoculation treatments were higher than those without inoculation, Significantly lower than the non-vaccinated control. The results of RDA analysis showed that the infection rates of AM fungi were significantly correlated with the content of available P (r = 0.65, P <0.05), phosphatase (r = 0.74, P < (R = -0.55, P <0.05), the amount of amylase (r = - 0.85, P <0.05) and the number of fungi 0.58, P <0.05), N (r = -0.77, P <0.05), β-glucosidase (r = -0.90, 0.05) was negatively correlated. Therefore, inoculation of AM fungi cucumber mycorrhizal plantation increased soil enzyme activity, optimize greenhouse cucumber rhizosphere microbial community structure, significantly improved soil physical and chemical properties.