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用福建漳州水仙(Narcissus tazetta L.var.Chinensis Roem)为实验材料,重复Benjamin等双鳞片培养。实验结果说明容易诱发小鳞茎,且在培养基中加入活性炭和进行暗培养,对小鳞茎的诱导和生长更为有利。通过小鳞茎诱导发生的细胞组织学观察,确实证明双鳞片培养中小鳞茎不通过愈伤组织而直接从鳞片腋间发生,这对水仙的种质保存比较有利。
Using Narcissus tazetta L.var. Chinensis Roem as experimental material, Benjamin and other double-flake cultures were repeated. The experimental results show that it is easy to induce bulblets, and adding activated carbon and dark culture to the medium is more beneficial to the induction and growth of bulblets. The observation of cell histology induced by bulblets did indeed prove that bulblets in double-flake cultures did not pass through the calluses but occurred directly from the axilla of the scales, which is advantageous for preservation of the nymphae.