The Establishment of an Enzyme-linked Immunosorbent Assay for Carcinoembryonic Antigen

• 出 处: Annual Report for China Institute of Atomic Energy
• 发表日期: 2001年00期
• 关键词: immunosorbent monoclonal Antigen peroxidase labeled coated Enzyme ranged

Two anti-CEA monoclonal antibodies are used, one is coated on the microtiter plate, the other is labeled with horseradish peroxidase(HRP). The two-step assay is established based on enzyme-linked immunosorbent assay(ELISA). TMB-H2O2 solution is used as the substrate of HRP. The sensitivity of the assay is 0.4 μ g/L. The intra-assay CVs and the inter-assay CVs are lower than 10.0% and 15.0%, respectively. The analytical recoveries are ranged from 99.4% to 108.7%. The reference cut-off value of normal serum (n= 100 ) is 10.0 ng/L.

Two anti-CEA monoclonal antibodies are used, one is coated on the microtiter plate, the other is labeled with horseradish peroxidase (HRP). The two-step assay is based on enzyme-linked immunosorbent assay The sensitivity of the assay is 0.4 μg / L. The intra-assay CVs and the inter-assay CVs are less than 10.0% and 15.0%, respectively. The analytical recoveries are ranged from 99.4% to 108.7%. The reference cut-off value of normal serum (n = 100) is 10.0 ng / L.