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目的:建立测定不同市售荷叶药材中金丝桃苷和异槲皮苷含量的高效液相色谱法。方法:采用Nova-Pak C18(3.9 mm×150 mm,5μm)色谱柱,以乙腈-0.1%甲酸水(13∶87)为流动相,流速1.0 mL.min-1,检测波长255 nm,柱温34℃。结果:金丝桃苷和异槲皮苷进样量分别在0.056～0.28μg和0.037～0.26μg范围内呈线性关系;平均回收率(n=6)分别为96.1%(RSD=2.7%)和98.8%(RSD=2.6%)。46批市售药材中金丝桃苷和异槲皮苷的含量分别为0.35%～1.47%和0.10%～0.69%。结论:该方法简便、准确,可作为荷叶药材中金丝桃苷和异槲皮苷的含量测定方法;市售荷叶药材中金丝桃苷和异槲皮苷的含量变化较大,应将其纳入荷叶药材的质量控制标准。
Objective: To establish a HPLC method for the determination of hyperoside and isoquercitrin in different lotus leaf medicinal materials. Methods: Nova-Pak C18 column (3.9 mm × 150 mm, 5 μm) was used with acetonitrile-0.1% formic acid (13:87) as the mobile phase at a flow rate of 1.0 mL · min- 34 ° C. RESULTS: The injections of hyperoside and isoquercitrin were linear in the range of 0.056-0.28μg and 0.037-0.26μg respectively. The mean recoveries (n = 6) were 96.1% (RSD = 2.7%) and 98.8% (RSD = 2.6%). The contents of hyperoside and isoquercitrin in 46 batches of medicinal herbs were 0.35% -1.47% and 0.10% -0.69%, respectively. Conclusion: The method is simple and accurate, and can be used as a method for the determination of hyperoside and isoquercitrin in lotus leaf. The contents of hyperoside and isoquercitrin in commercially available lotus leaf-medicinal materials vary greatly. Will be incorporated into lotus leaf quality control standards.