论文部分内容阅读
目的了解密云地区莱姆病宿主动物和传播媒介的感染情况,以及宿主动物的分布情况和传播媒介蜱的季节消长规律,为密云地区莱姆病的防制提供科学依据。方法采用布旗法捕蜱,用倒置显微镜观察蜱的形态,对蜱进行鉴定。采用夹夜法捕鼠;用BSKⅡ培养基对蜱和鼠标本进行病原分离。采用巢式聚合酶链反应(PCR)对蜱和鼠进行病原学检测。羊血清莱姆病抗体检测采用间接免疫荧光抗体法。结果密云地区捕获的蜱均为长角血蜱,其消长曲线具有明显的季节性,巢式PCR检测蜱带菌率为10.16%;黑线姬鼠和北社鼠是该地区优势鼠种,巢式PCR检测鼠带菌率为4.26%;当地放养山羊血清学检测阳性率为12.00%。结论在蜱和鼠中均检测到莱姆病螺旋体的特异片段,表明密云地区可能为莱姆病自然疫源地。
Objective To understand the infection status of Lyme disease hosts and vectors in Miyun area, as well as the distribution of host animals and seasonal fluctuation of vector ticks, and provide a scientific basis for the prevention and control of Lyme disease in Miyun area. Methods Bunting method was used to catch ticks. The shape of ticks was observed with inverted microscope and the ticks were identified. The method of catching the night with the trap method; using the BSKⅡmedium to separate the tick and the mouse pathogen. Ticks and mice were etiologically tested using nested polymerase chain reaction (PCR). Sheep serum Lyme disease antibody detection using indirect immunofluorescence antibody method. Results The ticks captured in Miyun area were all H. longicornis. The growth and decline curve of the ticks was obviously seasonal. The detection rate of ticks by nested PCR was 10.16%. The Apodemus agrarius and Beishekus were the dominant species in the area, The detection rate of PCR in mice was 4.26%; the positive rate of local stocking goats serological detection was 12.00%. Conclusion The specific fragments of Borrelia burgdorferi were detected in ticks and mice, indicating that Miyun may be the natural source of Lyme disease.