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目的从基因转录水平确定人视网膜色素上皮（ＲＰＥ）细胞是否具有合成纤溶酶原激活物抑制１（ＰＡＩ１）蛋白酶的生物学特性。方法取供体人眼ＲＰＥ细胞进行体外培养；当细胞生长融合后抽取ＲＮＡ，应用Ｎｏｔｈｅｒｎｂｌｏｔ分析技术，经特异的探针对人ＲＰＥ细胞的总ＲＮＡ进行检测。结果在体外培养的人ＲＰＥ细胞基因转录产物中存在有ＰＡＩ１ｍＲＮＡ的表达，其长度分别为３．０ｋｂ和２．３ｋｂ。结论人ＲＰＥ细胞能够合成ＰＡＩ１蛋白酶；它可能在一些致盲性眼病的病理发生过程中起到重要的作用。
Objective To determine whether the human retinal pigment epithelium (RPE) cells have the biological characteristics of the synthetic plasminogen activator inhibitor-1 (PAI-1) protease from the gene transcription level. Methods Donor human eye RPE cells were cultured in vitro. After the cells grew and fused, RNA was extracted and the total RNA of human RPE cells was detected by specific probe using Nothernblot assay. Results The expression of PAI-1 mRNA existed in human RPE cells cultured in vitro with length of 3.0kb and 2.3kb, respectively. Conclusions Human RPE cells can synthesize PAI-1 protease; it may play an important role in the pathogenesis of some blinding ophthalmias.