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真菌病原体已成为危害人类健康和农业的主要难题。作为一类真核细胞,真菌某些重要的生物特性与哺乳类动物的细胞雷同,以致现有的抗真菌抗生素往往不能区分真菌细胞和哺乳类动物细胞,导致严重的毒副作用。因此急需开发既有效、又安全的新型抗真菌药物。E1族大肠菌素是大肠杆菌产生的一种可形成离子通道的细菌素,它们只能杀伤大肠杆菌及亲缘关系接近的某些细菌。我们应用生物工程技术将白色念珠菌α交配信息素连接在大肠菌素Ia通道结构域上,即构成一种新型融合蛋白——信息菌素-CA(PMC-CA)。间接荧光标记数据显示该信息菌素可结合在真菌细胞上,实验室/田间实验数据证实信息菌素可抑制真菌生长。按分子量标化,田间实验数据证实信息菌素对稻瘟病的保护率显著高于三唑类、三环唑和稻瘟灵等现用抗稻瘟病药物的保护率。上述结果提示该融合蛋白具有发展成为农业抗真菌药物的价值。
Fungal pathogens have become a major challenge to human health and agriculture. As a group of eukaryotic cells, some of the important biological properties of fungi are similar to that of mammalian cells, so that existing antifungal antibiotics often fail to differentiate between fungal cells and mammalian cells, resulting in serious side effects. Therefore, the urgent need to develop both effective and safe new antifungal drugs. E1 colicin is produced by Escherichia coli to form ion channels of bacteriocins, they can only kill Escherichia coli and close to certain bacteria. We applied the bioengineering technique to connect Candida albicans α-mating pheromone to the coliforms Ia channel domain to form a novel fusion protein, creomycin-CA (PMC-CA). Indirect fluorescent labeling data showed that the signalin was able to bind to fungal cells, and lab / field experimental data confirmed that it inhibited fungal growth. According to the standardization of molecular weight, the data from field experiments confirmed that the protection rate of streptomycin against rice blast was significantly higher than that of the existing ones such as triazoles, tricyclazole and rice blast. The above results suggest that the fusion protein has the value of developing into an agricultural antifungal drug.