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目的比较蓝氏贾第鞭毛虫抗原快速检测法与多重PCR检测法检测结果间的相关性,为蓝氏贾第鞭毛虫快速筛查提供依据。方法采用抗原快速检测法与多重PCR检测法同时测定102例具有相关症状体征病人的粪便标本,确定病人蓝氏贾第鞭毛虫感染状况。结果共检测腹泻患者粪便标本102例,抗原快速检测法检测阳性81例,阴性21例,多重PCR检测阳性标本83例,阴性19例。抗原快速检测阳性而多重PCR检测确认阴性的标本0例;抗原快速检测阴性而多重PCR检测阳性的标本2例。抗原快速检测对蓝氏贾第鞭毛虫的阳性预示值为100.0%,阴性预示值为90.5%,诊断灵敏度是97.6%,诊断特异性是100.0%。结论采用抗原快速检测法检测蓝氏贾第鞭毛虫抗原,15 min内可筛查标本中的病原体,利于疾病的早期预防、诊断和治疗,防止社区/区域流行。
Objective To compare the correlation between the rapid detection of Giardia lamblia antigen and multiplex PCR detection and provide the basis for rapid screening of Giardia lamblia. Methods Rapid detection of antigen and multiplex PCR detection of 102 cases of patients with relevant signs and symptoms of stool specimens to determine the patient’s status of Giardia lamblia infection. Results A total of 102 cases of stool samples were detected in diarrhea patients. 81 cases were detected by antigen rapid test, 21 cases were negative, 83 cases were positive by multiplex PCR and 19 cases were negative. 0 cases were positive for rapid antigen detection and negative for multiple PCR test; 2 cases were negative for rapid antigen detection and positive for multiple PCR. The positive predictive value of rapid antigen detection against Giardia lamblia was 100.0%, the negative predictive value was 90.5%, the diagnostic sensitivity was 97.6% and the diagnostic specificity was 100.0%. Conclusion Rapid detection of antigen by antigen detection of Giardia lamblia antigens, 15 min can screen for specimens of pathogens, which will help the early prevention, diagnosis and treatment of the disease and prevent community / regional epidemic.