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本文用指纹法定量分析测定22种氨基酸,为前文“定性分离鉴定”的继续。定位显色剂采用镉—茚三酮酸化丙酮溶液,显色的最适温度及时间分别为50°—55℃及30分钟;再用甲醇洗脱30分钟,然后在波长为505nm处进行比色定量。本法测定氨基酸的灵敏度为10~(-6)—10~(-7)g,回收实验的误差在±10％以内。对8例正常人血清中16种游离氢基酸含量进行了定量分析测定,所得结果的范围与用离子交换柱或双向纸层析法相一致。本法可用于人体体液中游离氨基酸含量的微量定量分析,亦可用来测定蛋白质水解物的氨基酸含量。
In this paper, 22 kinds of amino acids were quantitatively analyzed by the fingerprint method, which is the continuation of the above-mentioned “qualitative isolation and identification”. Positioning chromogenic reagent using cadmium - ninhydrin acidification of acetone solution, the optimal temperature and time of the color development were 50 ° -55 °C and 30 minutes; then eluted with methanol for 30 minutes, and then at the wavelength of 505nm color comparison Quantitative. The sensitivity of this method to determine amino acids is 10~(-6)–10~(7)g, and the error of recovery experiments is within ±10%. The content of 16 free hydrogenic acids in serum of 8 normal persons was determined by quantitative analysis, and the range of the obtained results was consistent with ion exchange column or bidirectional paper chromatography. This method can be used for trace quantitative analysis of the free amino acid content in human body fluids, and can also be used to determine the amino acid content of protein hydrolysates.