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[目的]评价含凝血酶敏感素基序的去整合素金属蛋白酶1(a disintegrin and metalloproteinase with thrombospondin motifs 1,ADAMTS1)m RNA及其蛋白表达在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)发生发展中的意义。[方法 ]应用RT-PCR方法及免疫组织化学SP方法分别检测ESCC组织及癌旁正常组织中ADAMTS1 m RNA及其蛋白表达的情况。[结果]1ADAMTS1m RNA在ESCC组织中表达量显著性低于癌旁正常组织(0.394±0.123 vs 0.895±0.276,P<0.01);ADAMTS1m RNA在无淋巴结转移组中的表达量显著性低于淋巴结转移组(0.298±0.102 vs 0.482±0.157,P<0.05)。2ESCC组织中ADAMTS1蛋白的阳性表达率显著性低于癌旁组织(38.9%vs 94.4%,P<0.01)。[结论]ADAMTS1异常表达可能与ESCC的发生、发展及淋巴结转移密切相关。
[Objective] To evaluate the expression of a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) mRNA and its protein in esophageal squamous cell carcinoma (ESCC) The development of significance. [Methods] RT-PCR and immunohistochemistry SP method were used to detect the expression of ADAMTS1 m RNA and its protein in ESCC tissues and adjacent normal tissues respectively. [Results] The expression of 1ADAMTS1mRNA in ESCC tissues was significantly lower than that in adjacent normal tissues (0.394 ± 0.123 vs 0.895 ± 0.276, P <0.01). The expression of ADAMTS1mRNA in ESCC tissues was significantly lower than that in lymph node metastasis Group (0.298 ± 0.102 vs 0.482 ± 0.157, P <0.05). The positive expression rate of ADAMTS1 protein in 2ESCC tissues was significantly lower than that in paracancerous tissues (38.9% vs 94.4%, P <0.01). [Conclusion] The abnormal expression of ADAMTS1 may be closely related to the occurrence and development of ESCC and lymph node metastasis.