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OBJECTIVE Diabetic peripheral neuropathy(DPN)is the cause of considerable morbidity and mortality indiabetic patients.The loss of nerve fibersis the main pathological characteristics of the DPN and the pathway of Oct6-Krox20 plays an important role in the formation of myelin sheath.In our previous study,we found that Fuzi(Radix aconite lateral ispreparata)could significantly improve the nerve conduction deficits and thermal hypoalgesia deficits in the diabetic rats,but the underlying molecular mechanisms have not been established.The aim of this study is to investigate the expression of Oct6,Krox20,myelin basic protein(Mbp)and myelin protein zero(Mpz)in Schwann cells and analyze the effect of Fuzi in the formation of myelin sheath.METHODS There were six groups in the study.In the control group,thecells weresupplemented with normal cell culture medium.In the mannitol group,the cells were fed with normal glucose plus mannitol.In the model group,the cells were supplemented with high glucose medium(75 mmol·L-1).In the other group,the cells weretreated with high glucose medium(75mmol·L-1)plus different concentrations of Fuzi(0.1,1.0 and 10.0μg·mL-1).After three days,real-time PCR was used to detect gene expression.RESULTS Compared with the control group,the model group showed lowerexpression of Oct6,Krox20,Mbp and Mpz.In comparison to the model group,Fuzi(0.1,1.0and 10.0μg·mL-1)increased the expression of Oct6,Krox20,Mbp and Mpz.CONCLUSION These results demonstrate that Fuzi enhances the protein of myelin sheath through impacting the pathway of Oct6-Krox20.
OBJECTIVE Diabetic peripheral neuropathy (DPN) is the cause of considerable morbidity and mortality indiabetic patients. The loss of nerve fibersis the main pathological characteristics of the DPN and the pathway of Oct6-Krox20 plays an important role in the formation of myelin sheath.In our previous study, we found that Fuzi (Radix aconite lateral ispreparata) could significantly improved the nerve conduction deficits and thermal hypoalgesia deficits in the diabetic rats, but the underlying molecular mechanisms have not been established. The aim of this study is to investigate the expression of Oct6, Krox20, myelin basic protein (Mbp) and myelin protein zero (Mpz) in Schwann cells and analyze the effect of Fuzi in the formation of myelin sheath. METHODS There were six groups in the study. The control group, the cells weresupplemented with the normal cell culture medium. in the mannitol group, the cells were fed with normal glucose plus mannitol. the model group, the cells were supplemented with high gluco se medium (75 mmol·L-1) .In the other group, the cells weretreated with high glucose medium (75 mmol·L-1) plus different concentrations of Fuzi (0.1, 1.0 and 10.0 μg · mL- days, real-time PCR was used to detect gene expression .RESULTS Compared with the control group, the model group showed lowerexpression of Oct6, Krox20, Mbp and Mpz.In comparison to the model group, Fuzi (0.1, 1.0 and 10.0 μg · mL-1) increased the expression of Oct6, Krox20, Mbp and Mpz.CONCLUSION These results demonstrate that Fuzi enhances the protein of myelin sheath through impacting the pathway of Oct6-Krox20.