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目的探讨钒暴露与热休克蛋白(Heat Shock Protein,HSP)70-2基因多态性对作业工人神经行为功能的影响。方法采用整群抽样抽取某钒制品厂和某化工厂符合条件的各128例工人作为接钒组和对照组,用神经行为核心测验组合(NCTB)测试研究对象的神经行为功能;通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法来检测作业工人hsp70-2(rs1061581)位点的单核苷酸多态性。结果 (1)在hsp70-2位点上,接钒组和对照组在神经行为功能上的差异主要表现为:在A/G基因型组中,接钒组在错误次数、视觉保留、数字跨度(倒序)、提转捷度(非利手)、目标追踪(正确点数、总打点数)6项上得分更低且差异有统计学意义(P<0.05);而在A/A基因型组中,接钒组仅在数字跨度(倒序)上得分更低且差异有统计学意义(P<0.05);在G/G基因型组中,接钒组仅在错误次数、数字译码两项上得分更低且有统计学意义(P<0.05)。采用主成分分析法构建了神经行为综合指数(Neurobehavioral Index,NBI),在A/G基因型组中,接钒组的NBI得分更低且差异有统计学意义(P<0.05)。(2)接钒组在包括提转捷度(非利手),目标追踪(正确点数,总打点数),数字跨度(顺序)以及NBI得分均以带有A/G基因型的一组得分最低,但各基因型间差异均无统计学意义(P>0.05),对照组未发现此规律。(3)析因分析未发现钒暴露与hsp70-2多态性存在能影响调查对象神经行为功能的交互作用。结论在神经行为表现上,含有A/G基因型的个体在神经行为表现上更容易受到钒暴露的影响,尚未观察到hsp70-2与钒暴露间存在交互作用。
Objective To investigate the effects of vanadium exposure and heat shock protein 70-2 gene polymorphism on the neurobehavioral function of workers. Methods A total of 128 eligible workers in a vanadium product factory and a chemical factory were sampled by cluster sampling as the vanadium group and control group. The neurobehavioral function of the subjects was tested by the neurobehavioral core test combination (NCTB) Response-restriction fragment length polymorphism (PCR-RFLP) method to detect hsp70-2 (rs1061581) site single nucleotide polymorphisms. Results (1) At the hsp70-2 locus, differences in neurobehavioral function between the vanadium-receiving group and the control group were mainly as follows: in the A / G genotype group, the number of false positives, visual retention, (Reverse order), turn the turn (non profit), target tracking (correct points, the total number of RBI) 6 items lower and the difference was statistically significant (P <0.05); while in the A / A genotype group (P <0.05). In the G / G genotype group, the vanadium group received only the number of errors and number decoding Scored lower and statistically significant (P <0.05). The principal component analysis was used to construct the neurobehavioral index (NBI). In the A / G genotype group, the NBI scores in the vanadium group were lower and the difference was statistically significant (P <0.05). (2) The vanadium group received a set of scores with A / G genotypes in terms of turnaround (non-profit), target tracking (correct points, total number of RBIs), numerical span (order), and NBI scores The lowest, but no significant difference between the genotypes (P> 0.05), the control group did not find this rule. (3) Factorial analysis found no interaction between vanadium exposure and hsp70-2 polymorphism could affect the neurobehavioral function of the surveyed subjects. Conclusion In neurobehavioral manifestation, individuals with A / G genotype are more likely to be affected by vanadium exposure in neurobehavioral manifestation, and no interaction between hsp70-2 and vanadium exposure has been observed.