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幼儿急疹是婴儿常见病,病毒病因一直未被证实。1986年Salahuddin等分离出一种新的人疱疹病毒——人亲B淋巴细胞病毒-6(HBLV)随后,人们认为它就是人疱疹病毒(HHV-6)。本文以病毒分离和血清学方法研究了HHV-6和幼儿急疹的关系。方法病毒分离与培养:发热期采血,肝素抗凝Ficou-Paque液梯度离心,提取单核细胞,以RPMI-1640培养基(含RPMI-1640、10%胎牛血清、重组人白细胞介素2和PHA 5 um/ml)培养,置于CO_2孵箱、37℃培养。每周更换培养液并镜检。第9天收集细胞用冷丙酮固定,用急性期和恢复期病人血清作抗补体免疫荧光染色。HHV-6在脐带血细
Children with acute rash is a common infant disease, the etiology of the virus has not been confirmed. In 1986, Salahuddin et al. Isolated a new human herpes virus, human pro-B lymphocyte virus-6 (HBLV). Subsequently, it was thought to be human herpesvirus (HHV-6). In this paper, the relationship between HHV-6 and infant acute rash was studied by virus isolation and serological methods. Methods Isolation and culture of virus: Blood samples were collected during the fever period and mononuclear cells were extracted by Ficou-Paque heparin anticoagulation. The cells were cultured in RPMI-1640 medium containing RPMI-1640, 10% fetal bovine serum, PHA 5 um / ml), placed in CO 2 incubator, 37 ℃ culture. Weekly culture medium and microscopic examination. On the 9th day, the cells were harvested and fixed with cold acetone. Anti-complement immunofluorescence staining was performed with the serum of the acute and convalescent patients. HHV-6 in the umbilical cord blood thin