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目的:优选温阳活血颗粒的提取工艺。方法:采用HPLC测定芍药苷含量,流动相乙腈-0.1%磷酸水溶液(14∶86),检测波长230 nm;利用HPLC-ELSD测定人参皂苷Rg1,Re,Rb1含量,流动相乙腈(A)-水(B)梯度洗脱(0~35 min,19%A;35~55 min,19%~29%A;55~70 min,29%A;70~100 min,29%~40%A)。以干膏率和有效成分(人参皂苷Rg1,Re,Rb1和芍药苷)总含量的综合评分为评价指标,通过正交试验考察加水量、提取时间和提取次数对温阳活血颗粒提取工艺的影响。结果:最佳提取工艺为加8倍量水回流提取3次,每次1.5 h;干膏率依次为39.65%,人参皂苷Rg1,Re,Rb1和芍药苷提取量分别为3.49,2.79,6.64,218.60 mg。结论:优选的提取工艺科学合理、简便可行,适用于温阳活血颗粒的工业化生产。
Objective: To optimize the extraction process of Wenyang Huoxue granule. Methods: The content of paeoniflorin was determined by HPLC. The mobile phase was acetonitrile-0.1% phosphoric acid solution (14:86) and the detection wavelength was 230 nm. The contents of ginsenoside Rg1, Re and Rb1 were determined by HPLC-ELSD. (B) gradient elution (0-35 min, 19% A; 35-55 min, 19% -29% A; 55-70 min, 29% A; 70-100 min, 29-40% A). The dry weight and effective components (ginsenosides Rg1, Re, Rb1 and paeoniflorin) total score of the composite score as the evaluation index through orthogonal test to examine the amount of water, extraction time and the number of extraction on the Wenyanghuoxue particles extraction process . Results: The best extraction process was adding 8 times of water back to the extraction three times, each time being 1.5 h; the dry cream rates were 39.65%, the ginsenosides Rg1, Re, Rb1 and paeoniflorin were 3.49,2.79,6.64, 218.60 mg. Conclusion: The optimal extraction process is scientific and reasonable, simple and feasible, and suitable for the industrialized production of Wenyang Huoxue Granules.