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目的:探讨骨桥蛋白(OPN)与CD44v6分子mRNA在卵巢癌中的表达情况及其与卵巢肿瘤临床病理特征之间的关系,阐明OPN和CD44v6分子在卵巢癌侵袭和转移过程中的作用。方法:应用逆转录聚合酶链反应(RT-PCR)方法,检测OPN和CD44v6 mRNA在卵巢癌组织中的表达和相对含量,分析其与卵巢癌临床病理特征的关系。结果:OPN和CD44v6 mR-NA的表达与肿瘤临床分期、组织学分级、淋巴结转移与否具有统计学差异(P<0.05或P<0.01),与患者的年龄、肿瘤的病理类型无统计学差异(P>0.05);OPN和CD44v6间有显著相关性(r=0.35,P<0.01)。结论:OPN与CD44v6 mRNA的表达增高,在上皮性卵巢癌的发生、发展和侵袭转移过程中可能起重要作用;二者表达具有一致性,OPN表达增加可能使CD44v6表达上调。
OBJECTIVE: To investigate the expression of osteopontin (OPN) and CD44v6 mRNA in ovarian cancer and their relationship with the clinicopathological features of ovarian cancer, and to clarify the role of OPN and CD44v6 in invasion and metastasis of ovarian cancer. Methods: The expression and relative content of OPN and CD44v6 mRNA in ovarian cancer tissues were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), and their relationship with the clinicopathological features of ovarian cancer was analyzed. Results: The expression of OPN and CD44v6 mR-NA had no statistical significance with clinical stage, histological grade and lymph node metastasis (P <0.05 or P <0.01), but not with age and tumor pathological type (P> 0.05). There was a significant correlation between OPN and CD44v6 (r = 0.35, P <0.01). CONCLUSION: The increased expression of OPN and CD44v6 mRNA may play an important role in the occurrence, development and invasion and metastasis of epithelial ovarian cancer. The expression of OPN and CD44v6 mRNA may be up-regulated.