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采用RP-HPLC法测定肝毒净颗粒剂中岩白菜素的含量。固定相为Spherisorb-C18柱(10μm,4.6×250mm),流动相为甲醇-水(20:80),检测波长为275nm。岩白菜素的平均加样回收率为99.27%。
Determination of Bergenin in Ganjin Granules by RP-HPLC. The stationary phase was a Spherisorb-C18 column (10 μm, 4.6 × 250 mm). The mobile phase consisted of methanol-water (20:80) and the detection wavelength was 275 nm. The average recovery of bergenin was 99.27%.