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目的:探讨人参养荣汤对人胃癌裸小鼠化疗后血常规及骨髓有核细胞计数(BMC)的影响。方法:60只BALB/C裸雌小鼠采用前肢腋下接种人胃癌细胞SGC-7901建立人胃癌裸小鼠模型,接种后次日,将其随机分为阴性组,人参养荣汤低、中、高剂量组和模型组,每组12只。对除模型组外的其余组大鼠腹腔注射5-FU进行化疗,每周1次,共4周。同时对人参养荣汤低、中、高剂量组灌胃相应剂量人参养荣汤进行治疗。每天1次,共28d。血液细胞分析仪测定各组大鼠WBC、RBC、Hb、PLT,显微镜下进行骨髓有核细胞计数(BMC),比较测定结果。结果:与模型组比较,其余组血常规及BMC均升高(P<0.05,P<0.1),其中以人参养荣汤中剂量组升高最明显(P<0.05,P<0.01)。结论:人参养荣汤具有升高人胃癌裸小鼠血常规及BMC的作用,以临床等效剂量的中剂量作用效果最好。
Objective: To investigate the effect of ginseng and yangrong decoction on blood routine and bone marrow nucleated cell count (BMC) after chemotherapy in nude mice with gastric cancer. Methods: Sixty female BALB / C nude mice were inoculated with human gastric cancer SGC-7901 cells into the nude mouse model of BALB / C mice. The nude mice were randomly divided into the negative group, , High dose group and model group, 12 rats in each group. The rats in the other groups except the model group were intraperitoneally injected with 5-FU for once a week for 4 weeks. At the same time, ginseng yangrong soup low, medium and high dose group gavage the appropriate dose of ginseng yangrong soup for treatment. 1 day, a total of 28d. The WBC, RBC, Hb and PLT of rats in each group were measured by hematology analyzer, and the number of bone marrow nucleated cells (BMC) was counted under microscope. The results were compared. Results: Compared with the model group, the blood and BMC in the other groups were significantly increased (P <0.05, P <0.1). The most significant increase was seen in the medium dose group of Ginseng and Rongrong Decoction (P <0.05, P <0.01). Conclusion: Ginseng Yangrong Decoction has the effect of raising blood routine and BMC in human gastric cancer nude mice, and the effect is best with medium dose of clinical equivalent dose.