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利用银纳米粒子的表面增强拉曼散射(SERS)效应,研究了乙肝病毒表面抗原(HBsAg)和其鼠源单克隆抗体(单抗,Ab HBsAg)的相互作用.SERS光谱结果表明,Ab HBsAg分子主要通过位于非抗原结合部位的去质子化羧基(COO-)实现与银纳米粒子的结合.HBsAg与Ab HBsAg相互作用形成免疫复合物后,HBsAg分子上的色氨酸(Trp)残基特征振动完全消失,表明Trp残基位于HBsAg抗原分子的活性区,是HBsAg与Ab HBsAg相互作用的重要位点
The interaction between hepatitis B virus surface antigen (HBsAg) and its murine monoclonal antibody (mAb, Ab HBsAg) was studied by the surface enhanced Raman scattering (SERS) effect of silver nanoparticles.The results of SERS spectroscopy showed that Ab HBsAg molecule Binding to silver nanoparticles occurs mainly through the deprotonated carboxyl group (COO-) located at the non-antigen binding site.HBsAg interacts with Ab HBsAg to form an immunocomplex, and the tryptophan (Trp) residue on the HBsAg molecule vibrates Completely disappeared, suggesting that the Trp residue is located in the active region of the HBsAg antigen molecule and is an important site for the interaction between HBsAg and Ab HBsAg