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目的探讨中药抑抗汤含药血清对体外培养的小鼠子宫内膜容受性的影响。方法40只实验小鼠随机分为两组,分别用中药抑抗汤0.8ml和等量生理盐水灌胃28d,制备含药血清和空白血清。提取妊娠第4天(D4)雌鼠子宫内膜细胞后分为含药血清(A组)、胎牛血清(B组)和空白血清(C组)三组进行细胞培养。黏附实验检测子宫内膜细胞的黏附能力,ELISA法检测细胞培养液白血病抑制因子(LIF)水平,Western blot检测整合素ανβ3蛋白水平。结果 A组细胞黏附能力、LIF和整合素ανβ3蛋白表达均较B、C组显著增加(P<0.05)。结论抑抗汤可能是通过增强细胞黏附功能,增加LIF和整合素ανβ3的表达来改善子宫内膜容受性。
Objective To investigate the effect of Chinese medicine Kangqidang containing serum on endometrial receptivity in vitro of mice. Methods Forty experimental mice were divided into two groups at random: control group (0.8 mg) and saline (equal to normal saline) for 28 days respectively. Drug-containing serum and blank serum were prepared. Female uterine endometrial cells were extracted on day 4 of pregnancy (D4) and divided into three groups: drug-containing serum (group A), fetal bovine serum (group B) and blank serum (group C). Adhesion was used to detect the adhesion of endometrial cells. ELISA was used to detect the level of leukemia inhibitory factor (LIF) in the cell culture medium. Western blot was used to detect the level of integrin ανβ3. Results The cell adhesion ability, LIF and integrin ανβ3 protein expression in group A were significantly higher than those in group B and C (P <0.05). Conclusion YTD may improve endometrial receptivity by enhancing cell adhesion and increasing the expression of LIF and integrin αvβ3.