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以藏茴香不同海拔高度的叶片为试材,采用改良过的过氧化氢-醋酸离析法对其进行上、下表皮切片制作,番红染色,中性树胶封片,并在奥特光学BK-5000(OPTEC-BK5000)显微镜下观察比较,探讨叶表皮结构与环境因子的关系。结果表明:生长于不同海拔高度的藏茴香叶片,上表皮细胞为多边形,垂周壁平直、弓形,下表皮细胞形状为不规则形,垂周壁浅波状;气孔类型主要为不等细胞型,少数为横列型,散生于上、下表皮细胞当中;随海拔升高,上、下表皮细胞的形状无显著差别,气孔形状有长椭圆形与椭圆形2种;气孔随海拔升高而减小,向外突起程度增加,气孔密度随海拔升高总的来说呈增加趋势,只是在3 027~3 238m处略为减少,但二者之间没有显著差异。试验表明,这些特征是植物与环境相适应的结果。
The leaves of Tibetan anise at different altitudes were used as experimental materials. The upper and lower epidermis sections were made by using hydrogen peroxide-acetic acid method. 5000 (OPTEC-BK5000) under the microscope comparison to explore the relationship between leaf epidermal structure and environmental factors. The results showed that the upper epidermis cells were polygonal, the vertical epidermal cells were flat, and the shape of the arcuate and inferior epidermal cells was irregular with shallow wavy walls. The stomatal types were mainly of unequal cell types, As the horizontal type, scattered in the upper and lower epidermal cells among them; with the elevation, the shape of the upper and lower epidermal cells no significant difference in the shape of stomatal oblong and oval 2; stomatal decrease with increasing altitude , And the degree of outward protrusion increased. The stomatal density increased with the elevation generally, but decreased slightly at 3 027 ~ 3 238m, but there was no significant difference between the two. Experiments show that these characteristics are the result of plants adapted to the environment.