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【目的】在植物抗逆信号转导途径中,NAC转录因子处于承上启下的关键位置,通过调控靶基因的表达发挥抗逆功能。本研究克隆高粱SbNAC1基因全长cDNA,并对其进行序列比对、进化分析,为深入研究其在作物抗逆反应中的生物学功能奠定基础。【方法】RT-PCR扩增SbNAC1全长cDNA,并对其进行基因序列比对和系统进化分析等生物信息学分析。【结果】SbNAC1cDNA全长966bp,编码321个氨基酸。氨基酸序列比对结果表明高粱SbNAC1与水稻SNAC1同源性达到83.69%。
【Objective】 NAC transcription factor plays a pivotal role in plant resistance to signal transduction and regulates its expression by regulating its target genes. In this study, we cloned the full-length cDNA of SbNAC1 gene from sorghum and sequenced it for phylogenetic analysis, which laid the foundation for further study of its biological functions in crop resistance. 【Method】 The full-length cDNA of SbNAC1 was amplified by RT-PCR and bioinformatics analysis including sequence alignment and phylogenetic analysis. 【Result】 SbNAC1 cDNA was 966 bp in length and encoded 321 amino acids. The results of amino acid sequence alignment showed that the homology of sorghum SbNAC1 with rice SNAC1 was 83.69%.