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目的:观察慢性HIV-1感染者中CD4+和CD8+T淋巴细胞上BTLA(B and T lymphocyte attenuator,CD272)分子的表达及临床意义。方法:采集34例慢性HIV-1感染者和15例健康人的外周血,分离外周血单个核细胞,用间接免疫荧光染色方法标记并用流式细胞术检测T淋巴细胞上BTLA表达的百分比及平均荧光强度,并将BTLA的表达水平与患者CD4+T淋巴细胞计数和病毒载量做相关性分析。结果:与健康对照相比,HIV-1慢性感染者中CD4+和CD8+T细胞上的BTLA表达均明显下降,且BTLA的下降在各组HIV-1慢性感染者中也有明显差异,随着疾病进展,BTLA表达进行性下降。CD4+T细胞上BTLA表达比率与平均荧光强度均与CD4+T淋巴细胞计数呈明显正相关,而与病毒载量呈负相关。结论:在慢性HIV-1感染中,随着疾病进展,BTLA的进行性下降可能是机体活化和抑制信号失衡、导致免疫系统广泛活化的重要因素,恢复BTLA抑制信号的强度可能是治疗HIV感染的新策略。
Objective: To observe the expression and clinical significance of BTLA (B27 and T lymphocyte attenuator, CD272) on CD4 + and CD8 + T lymphocytes in chronic HIV-1 infected patients. Methods: Peripheral blood mononuclear cells from 34 chronic HIV-1 infected patients and 15 healthy volunteers were collected. Peripheral blood mononuclear cells (PBMCs) were isolated and labeled by indirect immunofluorescence staining. The percentage of BTLA expression on T lymphocytes was detected by flow cytometry Fluorescence intensity, and BTLA expression levels and CD4 + T lymphocyte count and viral load do correlation analysis. Results: Compared with healthy controls, the expression of BTLA on CD4 + and CD8 + T cells in HIV-1 chronic infection patients was significantly decreased, and the decrease of BTLA was also significantly different among HIV-1 chronic infection groups. With the disease Progress, BTLA expression decreased progressively. The ratio of BTLA expression and the average fluorescence intensity of CD4 + T cells were positively correlated with CD4 + T lymphocyte counts, but negatively correlated with viral load. CONCLUSIONS: In chronic HIV-1 infection, the progressive decline of BTLA as the disease progresses may be an important factor in the activation and suppression of signal imbalance in the body leading to extensive activation of the immune system, and the restoration of BTLA inhibitory signal may be the result of treatment of HIV-infected New strategy