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目的:评价三拗汤对哮喘小鼠变应性气道炎症的影响,并对全方成分进行分析。方法:BALB/c小鼠60只,随机分为正常组、模型组、三拗汤高、中、低剂量(7.2,3.6,1.8 g.kg-1)组、地塞米松组(0.75 mg.kg-1)。各组小鼠(除空白组)于第1,8天腹腔、皮下分别给予0.1 mL的致敏液(0.2 mL致敏液含卵蛋白OVA 0.1 mg,Al(OH)30.02 mg),第15～28天各组(除空白组)给予5%的OVA雾化,每次雾化20 min,每次雾化前30 min各组小鼠按体重给予相应的药物;空白组以等体积生理盐水代替致敏液,且生理盐水雾化相同的时间。相应的药物治疗2周后,取血做嗜酸细胞计数(EOS),取肺泡灌洗液(BALF)做细胞分类计数。采用超高效液相-四级杆串联飞行时间质谱(UPLC-QTOFMS)鉴定其化学成分。结果:三拗汤中剂量组使哮喘小鼠血中EOS的含量明显的降低(P<0.01),三拗汤高、低剂量组使哮喘小鼠血中的EOS含量有所降低(P<0.05),三拗汤高、中、低剂量使BALF中的EOS明显下降(P<0.01,P<0.05),从三拗汤全方中初步鉴定了22个化合物。结论:三拗汤对哮喘小鼠变应性气道炎症有明显的抑制作用,其全方主要化学成分为生物碱、黄酮、皂苷成分,这些化学组分与其发挥抗哮喘小鼠变应性气道炎症有密切的关系。
Objective: To evaluate the effect of San’ao Decoction on allergic airway inflammation in asthmatic mice and analyze its constituents. Methods: Sixty BALB / c mice were randomly divided into normal group, model group, Sanpo Tang high, medium and low dose groups (7.2,3.6,1.8 g.kg-1) and dexamethasone group (0.75 mg. kg-1). The mice in each group (except the blank group) were given intraperitoneal and subcutaneous 0.1 ml of sensitized solution (0.2 ml allergen OVA 0.1 mg, Al (OH) 30.02 mg) 28 days each group (except the blank group) given 5% OVA atomization, each atomized 20 min, 30 min before each atomized mice were given corresponding drug by body weight; blank group with an equal volume of saline instead Sensitized fluid, and the saline atomization of the same time. Two weeks after the corresponding drug treatment, blood was taken for eosinophil count (EOS), and bronchoalveolar lavage fluid (BALF) was taken for cell counting. The chemical constituents were identified by UPLC-QTOFMS using ultra-high performance liquid chromatography-quadrupole column time-of-flight mass spectrometry. Results: San’Ao Tang medium dose group decreased the content of EOS in the blood of asthmatic mice (P <0.01), and decreased the EOS content in asthmatic mice (P <0.05) ), Sanao Tang high, medium and low doses of EOS in BALF decreased significantly (P <0.01, P <0.05), from the San Anding Decoction of the initial identification of 22 compounds. Conclusion: Sanao decoction has obvious inhibitory effect on allergic airway inflammation in asthmatic mice. Its main chemical components are alkaloids, flavonoids and saponins, and these chemical constituents play an important role in allergic airway inflammation in asthmatic mice Road inflammation is closely related.