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本实验采用大鼠经额向基底池Willis环处插管注入NPY,NE和5-HT,以γCBF变化为指标,观察它们对脑血管的在体效应。实验发现NPY对脑血管具有很强的收缩作用,以克分子浓度计算NPY引起脑血管收缩比5-HT强10倍,比NE强500倍,并可增加NE,5-HT的缩血管效应。人及大鼠CSF中NPY测定发现:SAH伴有CVS的病人,CSF中NPY达291.1±58pg/ml,与对照组(164±28.00pg/ml)相比,P<0.01;大鼠SAH后CSF中NPY含量从2.1±2.1增至6.0±3.2ng/ml(P<0.01),同时大鼠皮层内NPY含量从9.5±0.8下降至4.8±0.8ng/ml(P<0.01),提示脑神经元分泌的NPY参与了CVS过程,并在维持CVS持续状态方面起重要作用。实验还提示SAH病人CSF中NPY含量变化可以作为观察CVS是否出现的指标。
In this study, NPY, NE and 5-HT were injected intratracheally into Willis ring of basal cistern, and the changes of γCBF were used as indexes to observe their in vivo effect on cerebrovascular. It was found that NPY had a strong contractile effect on cerebrovascular. NPY induced a vasoconstrictive effect of cerebral vasoconstriction 10-fold stronger than 5-HT and 500-fold stronger than NE, and increased vasoconstriction of NE and 5-HT. NPY in human and rat CSF was found to be 291.1 ± 58 pg / ml for NPY in CSF of patients with SAH associated with CVS, P <0.01 compared with that of the control group (164 ± 28.00 pg / ml) NPY content increased from 2.1 ± 2.1 to 6.0 ± 3.2 ng / ml (P <0.01), meanwhile NPY content decreased from 9.5 ± 0.8 to 4.8 ± 0.8 ng / ml in cortex (P <0.01), suggesting that neuronal Secreted NPY participates in the CVS process and plays an important role in maintaining the CVS persistence. The experiment also suggested that changes of NPY content in CSF of patients with SAH can be used as an index to observe whether CVS appears.