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目的:探讨吴茱萸次碱对大鼠重症急性胰腺炎(SAP)的疗效及其机制。方法:50只SD大鼠随机均分为假手术组和4个实验组,实验组大鼠以5%牛磺胆酸钠胰胆管逆行注射制作SAP模型后,分别给予生理盐水、乌司他丁、吴茱萸次碱、乌司他丁+吴茱萸次碱处理。术后24 h,行病理学检查,并检测各组血清淀粉酶活性及血浆与胰腺组织和内皮素1(EF-1)、降钙素基因相关肽(CGRP)水平。结果:除假手术组外,各实验组大鼠均胰腺组织均出现不同程度的胰腺炎病理改变;与假手术组比较,各实验组均出现不同程度的血清淀粉酶活性升高,血浆与胰腺组织ET-1浓度增加,而血浆与胰腺组织CGRP含量在吴茱萸次碱处理组与联合用药组均明显升高(均P<0.05),其余实验组无明显改变(均P>0.05);实验组中,各药物处理组胰腺病变程度、血清淀粉酶活性、血浆与胰腺组织ET-1浓度均较生理盐水处理的模型组明显降低,且均以联合用药组最为明显(均P<0.05)。结论:吴茱萸次碱对大鼠SAP具有治疗作用,其作用可能与增加CGRP水平从而改善胰腺组织微循环有关。
Objective: To investigate the effect and mechanism of rutaecarpine on severe acute pancreatitis (SAP) in rats. Methods: Fifty Sprague-Dawley rats were randomly divided into sham-operation group and four experimental groups. Rats in experimental group were treated with 5% sodium taurocholate and pancreaticoduodenectomy retrograde injection to make SAP model, then were given normal saline, ulinastatin , Rutaecarpine, ulinastatin + rutaecarpine treatment. The serum levels of amylase, plasma and pancreatic tissue, endothelin-1 (EF-1) and calcitonin gene-related peptide (CGRP) were measured 24 hours after operation. Results: In addition to the sham-operation group, pancreatic tissues of all experimental groups showed pathological changes of pancreatitis in varying degrees. Compared with the sham-operated group, serum amylase activity increased in all groups and plasma and pancreas (P <0.05). The levels of ET-1 in plasma and pancreas increased significantly in both the rutaecarpine group and the combination group (all P <0.05), while the other experimental groups showed no significant changes (all P> 0.05) , The degree of pancreatic lesions, the serum amylase activity, ET-1 concentration in plasma and pancreatic tissue in each drug-treated group were significantly lower than those in saline-treated group (all P <0.05). Conclusion: Rutaecarpine has a therapeutic effect on SAP in rats, which may be related to the increase of CGRP level and the improvement of pancreatic microcirculation.