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目的采用大鼠大脑中动脉梗死(MCAO)模型,评价不同时间窗尿激酶溶栓疗法疗效的差异。方法自体血栓法制作大鼠MCAO模型,90只大鼠随机分为6组。在栓塞后2h、4h、6h分别给予尿激酶及生理盐水。在溶栓前后2h、4h、6h各时点分别记录神经功能评分,TTC染色,计算梗死体积,光镜观察。结果神经功能评分,仅2h治疗组有显著性差异;脑梗死体积,2h和4h治疗组有显著性差异;光镜发现2h治疗组病理改变较轻。结论脑梗死后2h尿激酶治疗可减小梗死体积、改善神经功能。光镜观察发现病理改变最轻。
Objective To evaluate the curative effect of thrombolytic therapy with different time window on rat middle cerebral artery infarction (MCAO) model. Methods Rat MCAO model was made by auto thrombosis. 90 rats were randomly divided into 6 groups. After embolization 2h, 4h, 6h were given urokinase and normal saline. Neurological function scores, TTC staining, infarct volume and light microscope were recorded before and 2h, 4h, 6h after thrombolysis. Results The score of neurological function was significant only in 2h treatment group. The volume of cerebral infarction, 2h and 4h treatment group had significant difference. The pathological changes of 2h treatment group were found to be lighter by light microscope. Conclusion Urokinase treatment at 2 hours after cerebral infarction can reduce infarct volume and improve neurological function. Light microscope observation found that the lightest pathological changes.