Establishment of Multiplex RT-PCR Detection System for Three Viruses in Freesia

• 作 者: Fan Ronghui
• 机 构: InstituteofCropScience,FlowersResearchCenter,FujianEngineeringResearchCenterforCharacteristicFloricu
• 出 处: Plant Diseases and Pests(植物病虫害研究：英文版)
• 发表日期: 2013年1期
• 关键词: 病毒外壳蛋白 多重RT-PCR 检测系统 小苍兰 黄色花叶病毒 GENBANK 黄瓜花叶病毒 多重PCR Freesia Virus Freesia mosai
• 基金项目: Supported by Major Projects of Science and Technology Plan of Fujian Province （2010NZ0003） ＆ Finance Special Project of Fujian Province ＂Project of Sci-ence and Technology Innovation Team Building of

The specific primers were designed according to conserved sequences of coat protein (CP) gene of Freesia mosaic virus (FreMV), Cucumber mosaic virus (CMV) and Bean yellow mosaic virus (BYMV) published in GenBank, and a multiplex PCR protocol for simultane