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目的了解锦州市手足口病的病原学变化趋势,为手足口病防治工作提供参考依据。方法采集锦州市妇婴医院临床诊断为手足口病的患儿咽拭子和粪便标本,用实时荧光定量PCR仪对病毒进行分型。结果采集的717份样本中肠道通用病毒(EV)阳性682份,其中其他肠道病毒所占比例最高为44.13%,3年间肠道病毒阳性率差异无统计学意义(χ2=0.378,P>0.05);咽拭子和粪便样本的肠道病毒阳性率差异无统计学意义(χ2=0.894,P>0.05);共有15例手足口病患儿同时采集了咽拭子和粪便样本,检出结果完全相同,均为肠道病毒阳性。结论锦州市2012年以来儿童手足口病病原体以其他肠道病毒为主,与目前我国手足口病主要以EV71型为主不一致;手足口病患者的咽拭子和粪便标本的阳性检出率差异无统计学意义。
Objective To understand the etiological trend of hand-foot-mouth disease in Jinzhou city and provide a reference for the prevention and treatment of hand-foot-mouth disease. Methods Throat swabs and stool samples of children with hand-foot-mouth disease diagnosed in Maternal and Child Hospital of Jinzhou City were collected. The virus was typed by real-time fluorescence quantitative PCR instrument. Results Among the 717 samples collected, 682 samples were positive for enteric virus (EV), the highest proportion of other enteroviruses was 44.13%. There was no significant difference in the positive rate of enterovirus between the 3 years (χ2 = 0.378, P> 0.05). There was no significant difference in the positive rates of enterovirus between throat swabs and stool samples (χ2 = 0.894, P> 0.05). Throat swabs and stool samples were collected from 15 HFMD patients simultaneously The results are exactly the same, all positive for enteroviruses. Conclusion The pathogens of hand-foot-mouth disease in children in Jinzhou are mainly other enteroviruses since 2012, which is not consistent with the prevalence of hand-foot-mouth disease (EV71) in China. The positive rate of throat swab and stool specimens in hand-foot-mouth disease patients No statistical significance.