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为了解医院污水中肠道致病菌存在情况,我们于1989年对济南市各医院进行了沙门氏菌、志贺氏菌、EIEC、EPEC、ETEC、小肠结肠炎耶尔森氏菌和孤菌的检测工作。 沙门氏菌采用PBP前增菌和SFM、R_(19)两种选择性增菌法;经沉淀集菌分离培养。 耶尔森氏菌的检测:取样品100ml置无菌瓶中放4℃冰箱,增菌三周后,加入10％无水Na_2CO_30.4ml及10％Fe_2(SO_4)_30.35ml,摇匀后静置1小时取沉淀10ml加入10％KOH0.5ml,混匀立即接种SS平皿25℃培养24小时,分纯接种尿素和进
In order to understand the presence of enteric pathogens in hospital sewage, we conducted tests on Salmonella, Shigella, EIEC, EPEC, ETEC, Yersinia enterocolitica and Escherichia coli in Jinan hospitals in 1989 jobs. Salmonella using PBP pre-enrichment and SFM, R_ (19) two kinds of selective enrichment method; the precipitation of bacteria isolated and cultured. Yersinia detection: Take 100ml sample placed in a sterile bottle placed 4 ℃ refrigerator, after three weeks of enrichment, add 10% anhydrous Na_2CO_30.4ml and 10% Fe_2 (SO_4) _30.35ml, shake after the static Set 1 hour to take precipitate 10ml 10% KOH0.5ml, mix and immediately inoculate SS dish at 25 ℃ for 24 hours, were inoculated with urea and into