Preosteoblast-enriched Inc-Evf2 facilitates osteogenic differentiation by targeting Notch

• 作 者: Zhen Zhang Haixia Qi Han Xia Qi Liu Yi Ren Kun Zhang Yuan Xue Wei Hong
• 机 构: Department of Histology and Embryology,School of Basic Medical Sciences,Tianjin Medical University,T
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2021年2期
• 关键词: osteogenic differentiation IncRNA ossification of ligaments osteoporosis Notch

Ossification of ligaments(OL)and osteoporosis(OP)are multifactorial disorders without definitive clinical biomarkers.Long non-coding RNAs(IncRNAs)are known to involve in regulating pathogen-esis.Here,we have identified a preosteoblast-enriched Inc-Evf2 that was overexpressed in ossified ligamentum flavum(OLF)and down-expressed in OP.Inc-Evf2 is gradually upregulated during osteogenic induction,correlating with the enhanced expression of osteogenic marker genes and matrix mineralization.Moreover,knockdown of Inc-Evf2 significantly inhibits the expression of osteogenic differentiation markers and delays the osteoblastic mineralization process,indicating that this molecule is involved in osteogenesis.Mechanistically,we demonstrated that silencing of Inc-Evf2 decreases the protein level but not the mRNA levels of Notch2,Notch3,and Hes1,all of which correlate with osteogenesis.Taken together,our data demonstrate that Inc-Evf2 promotes osteogenic differentiation and bone formation through the Notch signaling,revealing that Inc-Evf2 may serve as a novel potential clinical target of OL and OP.