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应用聚合酶链反应（ＰＣＲ）和间接血凝法（ＩＨＡ）对６５３例肺炎患儿及２００例健康婴幼儿进行下呼吸道分泌物ＭＰＤＮＡ及血清抗ＭＰ－ＩｇＭ检测。结果表明：ＭＰＤＮＡ阳性１０７例，阳性率１６．４％；抗ＭＰ－ＩｇＭ阳性１２３例，阳性率１８．８％；两者同时阳性２３例，占阳性病例的１０％。ＭＰ阳性病例数共２０７例，占肺炎病例数的３１．７％。本实验结果提示应用ＰＣＲ技术对小儿ＭＰ肺炎的早期诊断具有特异、快速、敏感的特点，ＩＨＡ法检测抗ＭＰ－ＩｇＭ是病程消长患儿ＭＰ肺炎的诊断依据。
Polymerase chain reaction (PCR) and indirect hemagglutination (IHA) were performed on 653 children with pneumonia and 200 healthy infants for detection of lower respiratory tract secretions and serum anti-MP-IgM. The results showed that the positive rate of MPDNA in 107 cases was 16.4%. The positive rate of anti-MP-IgM in 123 cases was 18.8%. The positive rate of MPDNA in 23 cases was 10% of the positive cases. A total of 207 cases of MP-positive cases, accounting for 31.7% of the number of cases of pneumonia. The results suggest that the application of PCR technology for early diagnosis of pneumonia in children with MP has a specific, rapid and sensitive features, IHA method to detect anti-MP-IgM is the diagnosis of MP pneumonia in children with reduced disease duration.