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水稻品种广陆矮4号的干种子产经0、150、300和450Gy 的γ射线和0、0.1、0.2和0.3mmol 浓度的 EMS 复合处理后,从 M_1代生物学效应和 M_2代突变表明,M_1代的损伤效应随γ射线的剂量和 EMS 的浓度提高而增加。苗高、根长、苗高活力指数、细胞畸变率表现累加效应;根长活力指数和结实率存在着协合效应。复合处理的最佳剂量组合为300Gyγ射线与0.3mmol EMS 复合处理,其叶绿素突变、抽穗期和株高突变频率分别为4.02%、4.33%和4.05%,相互作用系数频率分别为1.03、1.31和1.27,表现为正的协合作用。
After the dry seeds of rice variety Guangluai 4 were treated with 0, 150, 300 and 450Gy gamma rays and 0, 0.1, 0.2 and 0.3 mmol EMS concentrations, the biological effects of M 1 generation and the M 2 generation mutation indicated that, The damage effect of M_1 generation increased with the dose of γ-ray and the concentration of EMS. Seedling height, root length, seedling vigor index, and cell aberration rate showed additive effect. Synergistic effect existed between root vigor index and seed setting rate. The optimal dose combination of composite treatment was 300 Gy γ-ray and 0.3 mmol EMS, the chlorophyll mutation, heading date and plant height mutation frequency were 4.02%, 4.33% and 4.05% respectively, and the interaction coefficients were 1.03, 1.31 and 1.27 respectively , Showing a positive synergistic effect.