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本实验利用高体孵育脑薄片和放射免疫测定精氨酸加压素（ＡＶＰ）的方法，初步探讨了牛血清白蛋白耦联皮质酮（Ｂ－ＢＳＡ，不易进入细胞内）对大鼠下丘脑薄片（含室旁核和视上核）释放ＡＶＰ的影响和可能机制。结果：（１）Ｂ－ＢＳＡ（１０－７─１０－４ｍｏｌ／Ｌ）在２０ｍｉｎ内对大鼠下丘脑薄片ＡＶＰ的释放具有明显的抑制性效应，且呈剂量一效应关系；（２）ＲＵ４８６（１０－４─１０－３ｍｏｌ／Ｌ）能部分地阻断Ｂ－ＢＳＡ的抑制效应；（３）Ｂ－ＢＳＡ的抑制效应随孵育液中Ｃａ２＋浓度的升高而明显增强；（４）在有新毒素（１０－３─１０－２ｍｏｌ／Ｌ）存在的情况下，Ｂ－ＢＳＡ的抑制效应显著增强。上述结果表明糖皮质激素在未进入细胞内的情况下亦可抑制大鼠下丘脑薄片释放ＡＶＰ。此作用发生在细胞膜水平上，由非基因组机制所介导，可能是影响Ｃａ２＋跨细胞膜流动的结果。
In this study, we investigated the effects of bovine serum albumin-conjugated corticosterone (B-BSA) on the hypothalamus Flakes (including paraventricular and supraoptic nuclei) affect the release of AVP and possible mechanisms. Results: (1) B-BSA (10-7─10-4 mol / L) had a significant inhibitory effect on the release of AVP in rat hypothalamus slices within 20 min, and showed a dose-response relationship; (2) RU486 10-4 ~ 10-3mol / L) partially blocked the inhibitory effect of B-BSA; (3) the inhibitory effect of B-BSA significantly increased with the increase of Ca2 + concentration in the incubation solution; (4) The inhibitory effect of B-BSA was significantly enhanced in the presence of toxin (10-3 ─ 10-2 mol / L). The above results indicate that glucocorticoids can also inhibit AVP release from rat hypothalamic slices without entering the cells. This effect occurs at the plasma membrane level and is mediated by non-genomic mechanisms that may be the result of Ca2 + flux across the cell membrane.