反相高效液相色谱法测定楤木属植物中黄酮类和香豆精的含量

• 作 者: 许旭东 张曙明 张聿梅 林耕 杨峻山
• 机 构: 中国医学科学院中国协和医科大学药用植物研究所
• 出 处: 药学学报
• 发表日期: 1999年01期
• 关键词: 反相高效液相色谱法 黄酮类化合物 香豆精

目的：对木属植物中草本和木本两个自然群进行化学分类学的研究。方法：应用反相高效液相色谱法测定木属植物中４种化学成分［ｎｅｏｌｉｇｕｉｒｉｔｉｎ（Ｉ），５，８ｄｉｍｅｔｈｏｘｙｃｏｕｍａｒｉｎ（ＩＩ），ｉｓｏｌｉｑｕｒｉｔｏｓｉｄｅ（ＩＩ），８［（２″，３″）ｐｒｅｎｙｌ］４′ｍｅｔｈｏｘｙｆｌａｖｏｎｅ７ＯβＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（２→１）αＬｒｈａｍｎｏｐｙｒａｎｏｓｉｄｅ（ＩＶ）］，对本属１１种植物中该４种成分进行了定量分析。色谱柱固定相为ＳｕｐｅｌｃｏＳＩＬＬＣ１８；流动相为４０％甲醇—甲醇梯度洗脱，根据４种成分紫外吸收波长的不同而改变检测波长；流速１０ｍｌ·ｍｉｎ－１；４种成分的线性范围分别为（０５６００～００１７５）μｇ，（０３７６０～００１１８）μｇ，（０２８７５～０００９０）μｇ和（０３０９０～０００９７）μｇ，回收率分别为９８９２％，９８２７％，９４７８％和９７４１％。结果：木本自然群中香豆精成分含量较高。结论：香豆精为木本自然群区别于草本自然群的特征成分之一

Objective: To study the chemical taxonomy of two natural populations of Herba Artemisiae arborescens. Methods: Four chemical constituents of Alnus were determined by reversed-phase high-performance liquid chromatography [neoliguiritin (I), 5,8-dimethoxycoumarin (II), isoliquritoside (II) Prenyl]  4’methoxyflavone7OβDglucopyranosyl (2 → 1) αLhamnopyranoside (IV)], the four components of 11 species of this genus were quantified analysis. The stationary phase of the column was SupelcoSIL-LC-18; the mobile phase was a gradient of 40% methanol-methanol. The detection wavelength was changed according to the UV absorption wavelength of the four components; the flow rate was 1.0 ml · min-1; The linear ranges were (05600 ~ 00175) μg, (03760 ~ 00118) μg, (02875 ~ 00090) μg and (03090 ~ 00097) μg, respectively. The recoveries Respectively, 9892%, 9827%, 9478% and 9741%. Results: The woody natural coumarin content is higher. Conclusion: Coumarin is one of the characteristic components that distinguish natural woody family from natural grassy group