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目的:建立药物生殖毒性研究中孕鼠离体子宫平滑肌张力的测定方法。方法:SD大鼠于妊娠第6~15天(GD6-15)给予受试物,在妊娠第20天(GD20)取子宫平滑肌,分别给予不同浓度缩宫素和硫酸镁溶液刺激,选择试验的最佳条件。在此基础上,测定不同剂量组孕鼠离体子宫平滑肌张力的变化情况,采用RM-6240BD多道生理信号采集处理系统分析数据,统计结果。结果:选择0.007 U/m L缩宫素、0.008 mol/m L硫酸镁为最佳刺激浓度。随着受试物剂量的增加,加入缩宫素后,各组妊娠子宫平滑肌的频率和张力均呈逐渐上升的趋势,而加入硫酸镁后,各剂量组妊娠子宫平滑肌的活动幅度、频率和张力均呈现降低的趋势,但各剂量组与溶媒对照组相比均未见明显差异(P>0.05)。结论:本研究建立了孕鼠离体子宫平滑肌张力的测定方法,该方法可以更好的反映受试物对孕鼠子宫肌的毒性作用,更加全面的评价受试物的生殖毒性。
Objective: To establish a method for the determination of uterine smooth muscle tension of pregnant rats in reproductive toxicity studies. Methods: The SD rats were given the test substance on the 6th to 15th days of gestation (GD6-15). Uterine smooth muscle was taken on the 20th day of gestation (GD20) and stimulated with different concentrations of oxytocin and magnesium sulfate solution respectively. Optimal conditions. On this basis, the change of uterine smooth muscle tension of pregnant rats in different dose groups was measured. The data and statistics results of multi-channel physiological signal acquisition and processing system of RM-6240BD were used. Results: Oxytocin (0.007 U / m L) and magnesium sulfate (0.008 mol / m L) were selected as the best stimulation concentration. With the increase of the dose of test substance, the frequency and tension of uterine smooth muscle in each group showed a gradual upward trend after adding oxytocin, while the activity, frequency and tension of uterine smooth muscle in each dose group after adding magnesium sulfate Showed a decreasing trend, but there was no significant difference between each dose group and vehicle control group (P> 0.05). Conclusion: This study established a method for the determination of uterine smooth muscle tension in pregnant rats. The method can better reflect the toxic effects of the test substance on the uterine muscle of pregnant rats and evaluate the reproductive toxicity of the test substance more comprehensively.